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Cells were lysed in buffer A (25 m m Tris, pH 7.5, 300 m m NaCl, 5% glycerol, 10 m m β-mercaptoethanol) using a microfluidizer. ... WT cells bearing ZapC- or mutant ZapC-GFP fusion plasmids were grown as described above and prepared for WesternA 600, ...

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03015.9064|8| TO RIGH T HAVING A RAD OF 300.00FT AND BEARING S11DEG 15MIN 03015.9064|9| 43SDS W WIT H AN ARC DIST OF 205.87FT TH S39DEG 25MIN 10SDS 03015.9064|10| E123.03FT TO BEG OF CURVE TO LEFT HAVING A RAD OF 250 ...

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A Fast Iterative Shrinkage-Thresholding Algorithm for Linear Inverse Problems | SIAM Journal on Imaging Sciences | Vol. 2, No. 1 | Society for .

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